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Protein Biochemistry
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KAPA HiFi HotStart ReadyMix
KAPA HiFi HotStart ReadyMix from Roche
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Citations:
(54)
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KAPA HiFi HotStart ReadyMix
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Description
HotStart ReadyMix, 500 x 25 µL reactions
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
KAPA HiFi HotStart ReadyMix
Company
Roche
Price
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Get Quote for this Product
Catalog Number
07958935001
Quantity
6.25 mL
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Roche
Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road
Indianapolis
,
Indiana
United States
Phone
: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911
Company Profile
Website:
sequencing.roche.com
Citations (54)
(1)
Cell-free multi-omics analysis reveals potential biomarkers in gastrointestinal cancer patients’ blood
Cell Reports Medicine
November 21, 2023
Yuhuan Tao, Shaozhen Xing, Shuai Zuo, Pengfei Bao, Yunfan Jin, Yu Li, Mingyang Li, Yingchao Wu, Shanwen Chen, Xiaojuan Wang,
et al.
Kit,
Qiagen
,Cat#
55284
Qubit dsDNA HS Assay kit
,
Thermo Fisher Scientific
,Cat#
Q32854
Kapa HiFi Hotstart ReadyMix
,
Roche
,Cat#
07958935001
Kapa
Hyper Prep
Kit,
Roche
,Cat#
07962363001
NEBNext
Multiplex
Oligos index,
NEB
,Cat#
E7335L
MagMeDIP-seq
(2)
Mural cell-derived chemokines provide a protective niche to safeguard vascular macrophages and limit chronic inflammation
Immunity
October 10, 2023
Kami Pekayvaz, Christoph Gold, Parandis Hoseinpour, Anouk Engel, Alejandro Martinez-Navarro, Luke Eivers, Raffaele Coletti, Markus Joppich, Flávio Dionísio, Rainer Kaiser,
et al.
samples were then again cleaned using cleanup beads (
1:1
beads/sample ratio). Using 1X KAPA HS Ready Mix (
07958935001
,
Roche
®) and 0.6 μM SINGV6 primer (IDT) in a 50 μL reaction synthesis of second strand and pre-
amplification
(3)
The largest HIV-1-infected T cell clones in children on long-term combination antiretroviral therapy contain solo LTRs
mBio
August 2, 2023
Johannes C. Botha, Dimiter Demirov, Carli Gordijn, Mary Grace Katusiime, Michael J. Bale, Xiaolin Wu, Daria Wells, Stephen H. Hughes, Mark F. Cotton, John W. Mellors,
et al.
were amplified by emulsion
PCR
in 20 µL reaction mixes with
KAPA HiFi HotStart ReadyMix
(2×) (
Roche
#
07958935001
), 100 ng of genomic DNA from PBMC, and 100 nM of each primer. The primers were designed with two phospho-thioester
(4)
Dietary restriction mitigates the age-associated decline in mouse B cell receptor repertoire diversity
Cell Reports
July 25, 2023
Carolina Monzó, Lisonia Gkioni, Andreas Beyer, Dario Riccardo Valenzano, Sebastian Grönke, Linda Partridge
proteins" ,,
Trizol Reagent
,
Thermo Fisher Scientific
,Cat#
15596018
2x
KAPA HiFi HotStart ReadyMix
,
KAPA Biosystems
,Cat#
07958935001
AMPure XP beads,
Beckman Coulter
,
A63882
,, Critical commercial
assays
,Critical commercial
assays
,Critical
(5)
Impact of supraphysiologic MDM2 expression on chromatin networks and therapeutic responses in sarcoma
Cell Genomics
July 12, 2023
Samantha M. Bevill, Salvador Casaní-Galdón, Chadi A. El Farran, Eli G. Cytrynbaum, Kevin A. Macias, Sylvie E. Oldeman, Kayla J. Oliveira, Molly M. Moore, Esmat Hegazi, Carmen Adriaens,
et al.
ligation
of barcoded
sequencing
adapters. Barcoded fragments were amplified via
PCR
(
KAPA HiFi
MM,
Roche
07958935001
).
ChIP-seq
was performed with a minimum of two biological replicates per
liposarcoma
cancer
line and
(6)
Microbiome remodeling through bacterial competition and host behavior enables rapid adaptation to environmental toxins
bioRxiv
June 28, 2023
Dan Kim, Olga Maria Pérez-Carrascal, Catherin DeSousa, Da Kyung Jung, Seneca Bohley, Lila Wijaya, Kenneth Trang, Sarah Khoury, Michael Shapira
Nextera XT
DNA library prep
kit (Illumina, FC-131–2001), and
KAPA HiFi
HotStart polymerase (
Roche
, #
07958935001
).
Amplification
was carried out with 95°C for 3min, 25 cycles of 95°C for 30sec, 55°C for 30sec and
(7)
Comprehensively defining cellular specializations for initiating parallel auditory pathways in the mouse cochlear nucleus
bioRxiv
June 9, 2023
Junzhan Jing, Ming Hu, Tenzin Ngodup, Qianqian Ma, Shu Ning Natalie Lau, Cecilia Ljungberg, Matthew J. McGinley, Laurence O. Trussell, Xiaolong Jiang
Next GEM Single cell 3’ Library Kit V3.1,10x Genomics,PN-1000157
Kapa HiFi HotStart ReadyMix
,
Roche
,
07958935001
Recombinant RNase Inhibitor,Takara,2313B Biocytin,Sigma-Aldrich,B4261 N-Methyl-D-glucamine,Sigma-Aldrich,M2004
Superscript II Reverse Transcriptase
(8)
The Effects of Paddy Cultivation and Microbiota Members on Arsenic Accumulation in Rice Grain
Foods
May 26, 2023
Esra Ersoy Omeroglu, Asli Bayer, Mert Sudagidan, Veli Cengiz Ozalp, Ihsan Yasa
V3 and V4 regions of the prokaryotic 16S rRNA regions with 2 ×
KAPA HiFi
HotStart Ready Mix (KK2602,
07958935001
,
Roche
, Germany) to give a total amplicon
PCR
mixture volume of 25 µL. The obtained forward sequences
(9)
Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq
Nature methods
May 1, 2023
Zou RS, Liu Y, Gaido OER, Konig MF, Mog BJ, Shen LL, Aviles-Vazquez F, Marin-Gonzalez A, Ha T.
manufacturer’s instructions. Dual-indexing
PCR
was performed using
KAPA HiFi HotStart ReadyMix
(
Roche
,
07958935001
) and
PCR
cleanup was performed using 1 × AMPure XP. Samples were quantified using QuBit (Thermo Fisher
(10)
Single duplex DNA sequencing with CODEC detects mutations with high sensitivity
Nature Genetics
May 1, 2023
Jin H. Bae, Ruolin Liu, Eugenia Roberts, Erica Nguyen, Shervin Tabrizi, Justin Rhoades, Timothy Blewett, Kan Xiong, Gregory Gydush, Douglas Shea,
et al.
M0269L
) at 30 °C for 20 min, followed by 0.75× AMPure XP cleanup; (5)
KAPA HiFi HotStart ReadyMix
(
Roche
,
07958935001
) and
xGen Library Amplification Primer Mix
(
IDT
, 1077677) were used for
PCR
by following the manual
(11)
RevGel-seq: instrument-free single-cell RNA sequencing using a reversible hydrogel for cell-specific barcoding
Scientific Reports
March 24, 2023
Jun Komatsu, Alba Cico, Raya Poncin, Maël Le Bohec, Jörg Morf, Stanislav Lipin, Antoine Graindorge, Hélène Eckert, Azadeh Saffarian, Léa Cathaly,
et al.
S1.(v)
Polymerase chain reaction
: 240 µL of the PCR supermix (1.66 ×
KAPA HiFi HotStart readymix
(
Roche
,
07958935001
), 1.33 µM
PCR
primer) is added to the 160 µL of the bead suspension. The beads in the reaction mix are
(12)
Epistasis lowers the genetic barrier to SARS-CoV-2 neutralizing antibody escape
Nature Communications
January 19, 2023
Leander Witte, Viren A. Baharani, Fabian Schmidt, Zijun Wang, Alice Cho, Raphael Raspe, Camila Guzman-Cardozo, Frauke Muecksch, Marie Canis, Debby J. Park,
et al.
tagmentation reaction was performed at 55 °C for 5 min. Next, 3.5 μl of
KAPA HiFi HotStart ReadyMix
(
Roche
,
07958935001
) and 1.25 μl of i5/i7 barcoded primers were added to the tagmented DNA to incorporate a unique indexing
(13)
Genome-wide profiling of histone modifications in Plasmodium falciparum using CUT&RUN
Life Science Alliance
January 1, 2023
Riward Campelo Morillo, Chantal T Harris, Kit Kennedy, Samuel R Henning, Björn FC Kafsack
pAG-MNase for ChIC/CUT&RUN,EpiCypher,EpiCypher,15-1016,15-1016
KAPA HIFI
HotStart ready mix,
Roche
,
Roche
,
07958935001
,
07958935001
AMPure XP beads,Beckman Coulter,
Beckman Coulter
,
A63881
,
A63881
Glycogen
for molecular biology;
(14)
Cell-free DNA methylation-defined prognostic subgroups in small-cell lung cancer identified by leukocyte methylation subtraction
iScience
December 22, 2022
Sami Ul Haq, Sabine Schmid, Mansi K. Aparnathi, Katrina Hueniken, Luna Jia Zhan, Danielle Sacdalan, Janice J.N. Li, Nicholas Meti, Devalben Patel, Dangxiao Cheng,
et al.
no.
28106
Kapa
Hyper Prep
Kit,
Roche
,Cat. no.
07962363001
KAPA HiFi HotStart ReadyMix
,
Roche
,Cat. no.
07958935001
Agencourt AMPure XP beads,
Beckman Coulter
,Cat. no.
A63881
MagMeDIP Kit,
Diagenode
,Cat. no.
C02010021
;
(15)
Longitudinal Analysis of the Microbiome and Metabolome in the 5xfAD Mouse Model of Alzheimer’s Disease
mBio
December 20, 2022
Sage J. B. Dunham, Katelyn A. McNair, Eric D. Adams, Julio Avelar-Barragan, Stefania Forner, Mark Mapstone, Katrine L. Whiteson
dev = 54.8 ng). Samples were prepared for
PCR
with
Kapa HiFi HotStart ReadyMix
(
Roche
, Cat. number
07958935001
) using the following primers: KAPA-
PCR
-F: 5′–AATGATACGGCGACCACCG*A−3′ and KAPA-
PCR
-R: 5′–CAAGCAGAAGACGGCATACG*A–3′.
PCR
(16)
The Maleth program: Malta's first space mission discoveries on the microbiome of diabetic foot ulcers
Heliyon
December 5, 2022
Christine Gatt, Braden T. Tierney, Pedro Madrigal, Christopher E. Mason, Afshin Beheshti, Anja Telzerow, Vladimir Benes, Graziella Zahra, Jurgen Bonett, Kevin Cassar,
et al.
assays
QIAamp® DNA Mini QIACube kit (240
),
QIAGEN
,Cat #
51326
KAPA HiFi HotStart ReadyMix
PCR kit
,
Roche
,Cat #
07958935001
4202 NEXTflexV1V3 kit,PerkinElmer,NOVA-4202-04 Agencourt AMPure XP Magnetic Beads,
Beckman Coulter
,
A63881
Deposited
(17)
Quantitative fate mapping: A general framework for analyzing progenitor state dynamics via retrospective lineage barcoding
Cell
November 4, 2022
Weixiang Fang, Claire M. Bell, Abel Sapirstein, Soichiro Asami, Kathleen Leeper, Donald J. Zack, Hongkai Ji, Reza Kalhor
Selective Antibiotic (G418 Sulfate),
ThermoFisher
,Cat#
10131035
KAPA HiFi HotStart ReadyMix
,
Roche
,Cat#
07958935001
KAPA
SYBR
FAST qPCR
Kits,
Roche
,Cat#
07959389001
Lipofectamine™
Stem
Transfection Reagent
,ThermoFisher,Cat#STEM00001 Matrigel
(18)
Mouse feeding study and microbiome analysis of sourdough bread for evaluation of its health effects
Frontiers in Microbiology
September 21, 2022
Joon-Gi Kwon, Sung-Hoon Park, Jeong-Eun Kwak, Jae Hyoung Cho, Gooyoun Kim, Deukbuhm Lee, Dong Hyun Kim, Hyeun Bum Kim, Ju-Hoon Lee
TAC HVG GGT ATC TAA TCC-3′). The
amplification
conditions using
KAPA HiFi
HotStart Ready Mix (Cat. #
07958935001
;
Roche
, Switzerland) were as follows: 1 cycle at 95°C for 3 min, 35 cycles at 95°C for 30 s, 35 cycles
(19)
High-throughput scNMT protocol for multiomics profiling of single cells from mouse brain and pancreatic organoids
STAR Protocols
September 16, 2022
Santiago Cerrizuela, Oguzhan Kaya, Lukas P.M. Kremer, Andrea Sarvari, Tobias Ellinger, Jannes Straub, Jan Brunken, Andrés Sanz-Morejón, Aylin Korkmaz, Ana Martín-Villalba
M DTT,Sigma-Aldrich,Cat#43816-10 mL
KAPA HiFi
HotStart Ready mix (2×),
Roche Diagnostics
,Cat#KK2602/
07958935001
KAPA HiFI
Hotstart PCR mix,
Roche Diagnostics
,Cat#KK2502/
07958897001
CT Conversion Reagent,
Zymo Research
,Cat#
D5003
-1 M-Solubilization
(20)
Response to immunotherapy in a patient with advanced epithelioid sarcoma of adrenal gland: A case report
Experimental and Therapeutic Medicine
September 7, 2022
Jingwen Wang, Chen Lu, Xi Tang
NEBNext Ultra II DNA Library Prep Kit for Illumina
,
E7645L
,
New England Biolabs
KAPA HiFi
HotStart Ready Mix,
07958935001
,
Roche Diagnostics
Capture,, SeqCap EZ Capture Beads,06977952001,Roche Diagnostics SeqCap EZ Accessory
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KAPA HiFi HotStart ReadyMix from Roche
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Product Specs
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